antibodies against esm1  (R&D Systems)

Journal: iScience

Article Title: Endothelial activating transcription factor 3 promotes angiogenesis and vascular repair in the mouse retina

doi: 10.1016/j.isci.2024.111516

Figure Lengend Snippet:

Article Snippet: Goat anti-ESM1 , R&D systems , Cat#: AF1999; RRID: AB_2101810.

Techniques: Recombinant, Transfection, Isolation, Gene Expression, Negative Control, Software

Journal: iScience

Article Title: Oncogenic HPV-induced high expression of ESM1 predicts poor prognosis and regulates aerobic glycolysis in cervical cancer

doi: 10.1016/j.isci.2024.110112

Figure Lengend Snippet:

Article Snippet: Rabbit anti-ESM1 , Huabio , Cat# ER62414; RRID: AB_3099428.

Techniques: Control, Recombinant, SYBR Green Assay, CCK-8 Assay, Lactate Assay, Glucose Assay, Luciferase, Enzyme-linked Immunosorbent Assay, Software

Journal: PLOS ONE

Article Title: Functional analysis of ESM1 by shRNA-mediated knockdown of its expression in papillary thyroid cancer cells

doi: 10.1371/journal.pone.0298631

Figure Lengend Snippet: A: The expression of ESM1 gene was higher in SW579, TPC-1 and B-CPAP cells than in Nthy-ori 3–1 cells. B: The endogenous screening experiments on shESM1 sequences designed with three different targets in TPC-1 cells showed that shESM1-3 had a higher knockdown efficiency on ESM1 than the control group. *P < 0.05, **P< 0.01, *** P <0.001.

Article Snippet: The membranes were hybridized with an anti-ESM1 antibody (cat. no. ab103590, Abcam) [ ] or an anti-GAPDH antibody (cat. no. 60004–1, Proteintech) [ ] overnight at 4˚C.

Techniques: Expressing

Journal: PLOS ONE

Article Title: Functional analysis of ESM1 by shRNA-mediated knockdown of its expression in papillary thyroid cancer cells

doi: 10.1371/journal.pone.0298631

Figure Lengend Snippet: A: The efficiency of TPC-1 cell infection by detecting the fluorescent signal of the GFP tag carried by the ESM1 knockout retroviral vector shESM1. B: After lentivirus infection, ESM1 expression in TPC-1 cells was significantly reduced in the shESM1 group compared with the shCtrl group. C: The results of Western blot showed that the ESM1 protein level of TPC-1 cells in the shESM1 group was down-regulated compared with the shCtrl group after retrovirus infection. D: The efficiency of SW579 cell infection by detecting the fluorescent signal of the GFP tag carried by the ESM1 knockout retroviral vector shESM1. E: After lentivirus infection, ESM1 expression in SW579 cells was significantly reduced in the shESM1 group compared with the shCtrl group. F: The results of Western blot showed that the ESM1 protein level of SW579 cells in the shESM1 group was down-regulated compared with the shCtrl group after retrovirus infection. **P< 0.01. images.

Article Snippet: The membranes were hybridized with an anti-ESM1 antibody (cat. no. ab103590, Abcam) [ ] or an anti-GAPDH antibody (cat. no. 60004–1, Proteintech) [ ] overnight at 4˚C.

Techniques: Infection, Knock-Out, Plasmid Preparation, Expressing, Western Blot

Journal: PLOS ONE

Article Title: Functional analysis of ESM1 by shRNA-mediated knockdown of its expression in papillary thyroid cancer cells

doi: 10.1371/journal.pone.0298631

Figure Lengend Snippet: Two-dimensional graph of flow cytometry showed that ESM1 knockdown significantly promoted apoptosis in TPC-1 cells.

Article Snippet: The membranes were hybridized with an anti-ESM1 antibody (cat. no. ab103590, Abcam) [ ] or an anti-GAPDH antibody (cat. no. 60004–1, Proteintech) [ ] overnight at 4˚C.

Techniques: Flow Cytometry

Journal: PLOS ONE

Article Title: Functional analysis of ESM1 by shRNA-mediated knockdown of its expression in papillary thyroid cancer cells

doi: 10.1371/journal.pone.0298631

Figure Lengend Snippet: A: Cell migration images of shCtrl group and shESM1 group in TPC-1 cells after ESM1 knockdown by scratch assay. B: In TPC-1 cells, the cell migration rate in the shESM1 group decreased by 83% at 6 h compared with the shCtrl group. C: Cell invasion and metastasis images of shCtrl group and shESM1 group in TPC-1 cells after ESM1 knockdown by transwell assay. D: In TPC-1 cells, the invasion and metastasis rate of the shESM1 group was 66% lower than that of the shCtrl group. **P< 0.01, *** P <0.001.

Article Snippet: The membranes were hybridized with an anti-ESM1 antibody (cat. no. ab103590, Abcam) [ ] or an anti-GAPDH antibody (cat. no. 60004–1, Proteintech) [ ] overnight at 4˚C.

Techniques: Migration, Wound Healing Assay, Transwell Assay

Journal: PLOS ONE

Article Title: Functional analysis of ESM1 by shRNA-mediated knockdown of its expression in papillary thyroid cancer cells

doi: 10.1371/journal.pone.0298631

Figure Lengend Snippet: A: Cell migration images of shCtrl group and shESM1 group in SW579 cells after ESM1 knockdown by scratch assay. B: In SW579 cells, the cell migration rate of the shESM1 group decreased by 34% at 24 h compared with the shCtrl group. C: Cell invasion and metastasis images of shCtrl group and shESM1 group in SW579 cells after ESM1 knockdown by transwell assay. D: In SW579 cells, the invasion and metastasis rate of the shESM1 group decreased by 58% compared with the shCtrl group. *P < 0.05, *** P <0.001.

Article Snippet: The membranes were hybridized with an anti-ESM1 antibody (cat. no. ab103590, Abcam) [ ] or an anti-GAPDH antibody (cat. no. 60004–1, Proteintech) [ ] overnight at 4˚C.

Techniques: Migration, Wound Healing Assay, Transwell Assay

Journal: PLOS ONE

Article Title: Functional analysis of ESM1 by shRNA-mediated knockdown of its expression in papillary thyroid cancer cells

doi: 10.1371/journal.pone.0298631

Figure Lengend Snippet: A: The expression of ESM1 gene was higher in SW579, TPC-1 and B-CPAP cells than in Nthy-ori 3–1 cells. B: The endogenous screening experiments on shESM1 sequences designed with three different targets in TPC-1 cells showed that shESM1-3 had a higher knockdown efficiency on ESM1 than the control group. *P < 0.05, **P< 0.01, *** P <0.001.

Article Snippet: The tissue sections were incubated with anti-ESM1 rabbit polygonal antibody (cat. no. ab103590, Abcam) overnight at 4˚C.

Techniques: Expressing

Journal: PLOS ONE

Article Title: Functional analysis of ESM1 by shRNA-mediated knockdown of its expression in papillary thyroid cancer cells

doi: 10.1371/journal.pone.0298631

Figure Lengend Snippet: A: The efficiency of TPC-1 cell infection by detecting the fluorescent signal of the GFP tag carried by the ESM1 knockout retroviral vector shESM1. B: After lentivirus infection, ESM1 expression in TPC-1 cells was significantly reduced in the shESM1 group compared with the shCtrl group. C: The results of Western blot showed that the ESM1 protein level of TPC-1 cells in the shESM1 group was down-regulated compared with the shCtrl group after retrovirus infection. D: The efficiency of SW579 cell infection by detecting the fluorescent signal of the GFP tag carried by the ESM1 knockout retroviral vector shESM1. E: After lentivirus infection, ESM1 expression in SW579 cells was significantly reduced in the shESM1 group compared with the shCtrl group. F: The results of Western blot showed that the ESM1 protein level of SW579 cells in the shESM1 group was down-regulated compared with the shCtrl group after retrovirus infection. **P< 0.01. images.

Article Snippet: The tissue sections were incubated with anti-ESM1 rabbit polygonal antibody (cat. no. ab103590, Abcam) overnight at 4˚C.

Techniques: Infection, Knock-Out, Plasmid Preparation, Expressing, Western Blot

Journal: PLOS ONE

Article Title: Functional analysis of ESM1 by shRNA-mediated knockdown of its expression in papillary thyroid cancer cells

doi: 10.1371/journal.pone.0298631

Figure Lengend Snippet: Two-dimensional graph of flow cytometry showed that ESM1 knockdown significantly promoted apoptosis in TPC-1 cells.

Article Snippet: The tissue sections were incubated with anti-ESM1 rabbit polygonal antibody (cat. no. ab103590, Abcam) overnight at 4˚C.

Techniques: Flow Cytometry

Journal: PLOS ONE

Article Title: Functional analysis of ESM1 by shRNA-mediated knockdown of its expression in papillary thyroid cancer cells

doi: 10.1371/journal.pone.0298631

Figure Lengend Snippet: A: Cell migration images of shCtrl group and shESM1 group in TPC-1 cells after ESM1 knockdown by scratch assay. B: In TPC-1 cells, the cell migration rate in the shESM1 group decreased by 83% at 6 h compared with the shCtrl group. C: Cell invasion and metastasis images of shCtrl group and shESM1 group in TPC-1 cells after ESM1 knockdown by transwell assay. D: In TPC-1 cells, the invasion and metastasis rate of the shESM1 group was 66% lower than that of the shCtrl group. **P< 0.01, *** P <0.001.

Article Snippet: The tissue sections were incubated with anti-ESM1 rabbit polygonal antibody (cat. no. ab103590, Abcam) overnight at 4˚C.

Techniques: Migration, Wound Healing Assay, Transwell Assay

Journal: PLOS ONE

Article Title: Functional analysis of ESM1 by shRNA-mediated knockdown of its expression in papillary thyroid cancer cells

doi: 10.1371/journal.pone.0298631

Figure Lengend Snippet: A: Cell migration images of shCtrl group and shESM1 group in SW579 cells after ESM1 knockdown by scratch assay. B: In SW579 cells, the cell migration rate of the shESM1 group decreased by 34% at 24 h compared with the shCtrl group. C: Cell invasion and metastasis images of shCtrl group and shESM1 group in SW579 cells after ESM1 knockdown by transwell assay. D: In SW579 cells, the invasion and metastasis rate of the shESM1 group decreased by 58% compared with the shCtrl group. *P < 0.05, *** P <0.001.

Article Snippet: The tissue sections were incubated with anti-ESM1 rabbit polygonal antibody (cat. no. ab103590, Abcam) overnight at 4˚C.

Techniques: Migration, Wound Healing Assay, Transwell Assay

Journal: Journal of Translational Medicine

Article Title: ANGPTL4 accelerates ovarian serous cystadenocarcinoma carcinogenesis and angiogenesis in the tumor microenvironment by activating the JAK2/STAT3 pathway and interacting with ESM1

doi: 10.1186/s12967-023-04819-8

Figure Lengend Snippet: The expression, potential functions, diagnostic value and prognostic value of ARGs in OC patients. A The mRNA expression of ARGs in OC samples compared to normal ovary samples based on the TCGA database. B The diagnostic value of ARGs for OC patients. C The PPI network for ARGs. D The correlation among these ARGs in OC patients. E GO and F KEGG enrichment analysis for these ARGs. G ANGPTL4 protein expression in OC and normal ovaries based on the HPA database. H The prognostic value of ANGPTL4 for OC patients based on the TCGA database. I The expression of ANGPTL4 in normal ovary samples and benign and malignant ovarian disease samples. J GSEA of ANGPTL4 based on the TCGA database. K Immune infiltration analysis for OC patients according to ANGPTL4 expression. L The expression of ANGPTL4 in multiple OC cell lines. M The expression of ESM1 and ANGPTL4 in multiple OC cell lines by Western blot. *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: The primary antibodies used are shown below: ANGPTL4 (Abcam, ab196746), ESM1 (Abcam, ab103590) and CD34 (Abcam, ab81289).

Techniques: Expressing, Diagnostic Assay, Western Blot

Journal: Journal of Translational Medicine

Article Title: ANGPTL4 accelerates ovarian serous cystadenocarcinoma carcinogenesis and angiogenesis in the tumor microenvironment by activating the JAK2/STAT3 pathway and interacting with ESM1

doi: 10.1186/s12967-023-04819-8

Figure Lengend Snippet: The expression of ANGPTL4 in ovarian cancer based on TCGA database

Article Snippet: The primary antibodies used are shown below: ANGPTL4 (Abcam, ab196746), ESM1 (Abcam, ab103590) and CD34 (Abcam, ab81289).

Techniques: Expressing

Journal: Journal of Translational Medicine

Article Title: ANGPTL4 accelerates ovarian serous cystadenocarcinoma carcinogenesis and angiogenesis in the tumor microenvironment by activating the JAK2/STAT3 pathway and interacting with ESM1

doi: 10.1186/s12967-023-04819-8

Figure Lengend Snippet: ANGPTL4 interacted with ESM1. A The correlation expression of ESM1 and ANGPTL4 in OC samples based on TCGA database. B IF staining for ANGPTL4 and ESM1 in OC tissue samples, SKOV3 cell lines, HeyA8 cell lines and HUVECs. C The interaction of ANGPTL4 and ESM1 in HeyA8 cells by Co-IP analysis. D GST pull-down analysis for GST-ANGPTL4 and His-ESM1. E Molecular docking for ANGPTL4 and ESM1 by HADDOCK.

Article Snippet: The primary antibodies used are shown below: ANGPTL4 (Abcam, ab196746), ESM1 (Abcam, ab103590) and CD34 (Abcam, ab81289).

Techniques: Expressing, Staining, Co-Immunoprecipitation Assay

Journal: Journal of Translational Medicine

Article Title: ANGPTL4 accelerates ovarian serous cystadenocarcinoma carcinogenesis and angiogenesis in the tumor microenvironment by activating the JAK2/STAT3 pathway and interacting with ESM1

doi: 10.1186/s12967-023-04819-8

Figure Lengend Snippet: ESM1 promotes ANGPTL4 to combine with LPL by accelerating proliferation, invasion and lipid accumulation in OC cells. A Molecular docking for ANGPTL4, LPL and ESM1 by HADDOCK. B Co-IP showed the effects of ESM1 on the interaction between ANGPTL4 and LPL in HeyA8 and SKOV3 cells. The effect of vector, ANGPTL4, ANGPTL4 + ESM1, ANGPTL4 KD, and ANGPTL4 KD + ESM1 on Hey-A8 and SKOV3 cell lipid levels and proliferation ability via oil red O staining ( C ), MTT ( D ) and EdU ( E ) analysis. The migration and invasion abilities were measured by wound healing ( F ) and Transwell assays ( G ) in Hey-A8 and SKOV3 cells transfected with vector, ANGPTL4, ANGPTL4 + ESM1, ANGPTL4 KD, and ANGPTL4 KD + ESM1. *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: The primary antibodies used are shown below: ANGPTL4 (Abcam, ab196746), ESM1 (Abcam, ab103590) and CD34 (Abcam, ab81289).

Techniques: Co-Immunoprecipitation Assay, Plasmid Preparation, Staining, Migration, Transfection

Journal: Journal of Translational Medicine

Article Title: ANGPTL4 accelerates ovarian serous cystadenocarcinoma carcinogenesis and angiogenesis in the tumor microenvironment by activating the JAK2/STAT3 pathway and interacting with ESM1

doi: 10.1186/s12967-023-04819-8

Figure Lengend Snippet: ESM1 inhibits ANGPTL4 binding to integrin α5β1 and VE-cadherin, which represses HUVEC proliferation and migration to induce vascular permeability in the tumor microenvironment. A Co-IP showed that ESM1 inhibited ANGPTL4 binding to α5β1 and VE-cadherin in HUVECs cultured with HeyA8 and SKOV3 cells, respectively. The effect of vector, ANGPTL4, ANGPTL4 + ESM1, ANGPTL4 KD, and ANGPTL4 KD + ESM1 on HUVECs cultured with Hey-A8 and SKOV3 cell lipid levels and proliferation ability via oil red O staining ( B ), MTT ( C ) and EdU ( D ) analysis. The migration ability was measured by wound healing ( F ) and Transwell assays ( G ) in HUVECs cultured with Hey-A8 and SKOV3 cells transfected with vector, ANGPTL4, ANGPTL4 + ESM1, ANGPTL4 KD, and ANGPTL4 KD + ESM1. *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: The primary antibodies used are shown below: ANGPTL4 (Abcam, ab196746), ESM1 (Abcam, ab103590) and CD34 (Abcam, ab81289).

Techniques: Binding Assay, Migration, Permeability, Co-Immunoprecipitation Assay, Cell Culture, Plasmid Preparation, Staining, Transfection

Journal: Journal of Translational Medicine

Article Title: ANGPTL4 accelerates ovarian serous cystadenocarcinoma carcinogenesis and angiogenesis in the tumor microenvironment by activating the JAK2/STAT3 pathway and interacting with ESM1

doi: 10.1186/s12967-023-04819-8

Figure Lengend Snippet: A model of the molecular mechanism by which ANGPTL4 promotes OC development and progression. A OC is a type of tumor with a rich blood supply. B There are many angiogenic factors in the tumor microenvironment of OC. C A variety of cells in the tumor microenvironment can take up these angiogenesis factors in a variety of ways to produce different pathophysiological effects. D ANGPTL4 can promote the proliferation and migration of OC cells by activating the JAK-STAT3 pathway, and ESM1 can bind ANGPTL4 and stabilize the binding of ANGPTL4 to LPL, thus promoting lipid accumulation and accelerating the proliferation and migration of OC cells. ANGPTL4 can also promote endothelial cell proliferation and migration through the JAK-STAT3 signaling pathway. ESM1 can inhibit the binding of ANGPTL4 to integrin α5β1 to increase vascular stability. Moreover, ESM1 promotes angiogenesis in the ovarian cancer microenvironment by binding to ANGPTL4 to inhibit its destruction of VE-cadherin-mediated tight junctions. E The molecular mechanism might be attributed to the interaction of ESM1 and ANGPTL4.

Article Snippet: The primary antibodies used are shown below: ANGPTL4 (Abcam, ab196746), ESM1 (Abcam, ab103590) and CD34 (Abcam, ab81289).

Techniques: Migration, Binding Assay